Detection of virus particles by scattering field using the multiperspective polarization modulation imaging method

Guo, B. et al. (2021) Detection of virus particles by scattering field using the multiperspective polarization modulation imaging method. Journal of the Optical Society of America B: Optical Physics, 38(12), pp. 3592-3600. (doi: 10.1364/JOSAB.436357)

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Abstract

The polarization parametric indirect microscopic imaging (PIMI) method, which employs a polarization-modulated incidence illumination and fitting the far-field variation of polarization states of scattered photons, is capable of direct identification of subdiffraction-scale structures and substances, such as virus particles. However, in the present strategy, the optical elements that collect the scattered photons are nearly fixed above the sample, making the collected information relatively limited, as the side-scattering photons are not fully utilized. To address this problem, we propose a multiperspective PIMI imaging method to maximize the collection of scattering photons from different spatial directions, which can obtain more information of optical anisotropy among particles. As a proof-of-concept study, virus detection using such a method is performed theoretically and experimentally. Results reveal that the virus particles can be detected and determined more distinctly thanks to the set of PIMI images from different spatial angles, showing notable superiority to the previous scheme, where only a plane PIMI image is derived from a fixed spatial direction. With the capability of acquiring more characteristics of the samples, the proposed multiperspective PIMI method can be applied in many fields, such as morphological characterization and biosensing.

Item Type:Articles
Additional Information:Funding.:National Natural Science Foundation of China (62105155, 61627802); Natural Science Foundation of Jiangsu Province (BK20210326); Engineering and Physical Sciences Research Council (EP/R042578/1); Ministry of Education Collaborative Project (B17023); Fundamental Research Funds for the Central Universities (30920010011); Beijing Municipal Natural Science Foundation (Z190018); Postdoctoral Science Foundation of Jiangsu Province (2020Z331); National Key Scientific
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liu, Professor Xuefeng and Marsh, Professor John and Hou, Dr Lianping
Authors: Guo, B., Ni, B., Jin, X., Zhang, H., Zhao, H., Hou, L., Marsh, J. H., Dong, L., Li, S., Xiong, J., and Liu, X.
College/School:College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
Journal Name:Journal of the Optical Society of America B: Optical Physics
Publisher:Optical Society of America
ISSN:0740-3224
ISSN (Online):1520-8540
Published Online:15 October 2021

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