Correlative imaging of trace elements and intact molecular species in a single-tissue sample at the 50 μm scale

de Jesus, J. M. et al. (2021) Correlative imaging of trace elements and intact molecular species in a single-tissue sample at the 50 μm scale. Analytical Chemistry, 93(40), pp. 13450-13458. (doi: 10.1021/acs.analchem.1c01927) (PMID:34597513)

Abstract

Elemental and molecular imaging play a crucial role in understanding disease pathogenesis. To accurately correlate elemental and molecular markers, it is desirable to perform sequential elemental and molecular imaging on a single-tissue section. However, very little is known about the impact of performing these measurements in sequence. In this work, we highlight some of the challenges and successes associated with performing elemental mapping in sequence with mass spectrometry imaging. Specifically, the feasibility of molecular mapping using the mass spectrometry imaging (MSI) techniques matrix-assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI) in sequence with the elemental mapping technique particle-induced X-ray emission (PIXE) is explored. Challenges for integration include substrate compatibility, as well as delocalization and spectral changes. We demonstrate that while sequential imaging comes with some compromises, sequential DESI-PIXE imaging is sufficient to correlate sulfur, iron, and lipid markers in a single tissue section at the 50 μm scale.

• Item Type: Articles
• Additional Information: This study was funded by an EPSRC strategic equipment award (EP/P001440/1) and an EPSRC sponsored fellowship (EP/R031118/1), the Surrey-NPL studentship fund. Beam time was provided by EPSRC National Research Facility UKNIBC NS/A000059/1 and RADIATE (Horizon 2020, grant agreement No 824096, proposal 19001813-ST). S
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Goodwin, Dr Richard
• Authors: de Jesus, J. M., Costa, C., Burton, A., Palitsin, V., Webb, R., Taylor, A., Nikula, C., Dexter, A., Kaya, F., Chambers, M., Dartois, V., Goodwin, R. J.A., Bunch, J., and Bailey, M. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Analytical Chemistry
• Publisher: American Chemical Society
• ISSN: 0003-2700
• ISSN (Online): 1520-6882
• Published Online: 01 October 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Analytical Chemistry 93(40): 13450-13458
• Publisher Policy: Reproduced under a Creative Commons License