Quercetin metabolites and protection against peroxynitrite-induced oxidative hepatic injury in rats

Yokoyama, A., Sakakibara, H., Crozier, A., Kawai, Y., Matsui, A., Terao, J., Kumazawa, S. and Shimoi, K. (2009) Quercetin metabolites and protection against peroxynitrite-induced oxidative hepatic injury in rats. Free Radical Research, 43(10), pp. 913-921. (doi: 10.1080/10715760903137010)

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Abstract

Quercetin has strong antioxidant potency. Quercetin-3′-O-sulphate (Q3′S) and quercetin-3-O-glucuronide (Q3GA) are the main circulating metabolites after consumption of quercetin-O-glucoside-rich diets by humans. However, information about how these quercetin metabolites function in vivo is limited. Hence, this study evaluated the efficacy of Q3′S and Q3GA for the protection of oxidative injury using in vitro and in vivo experiments. Peroxynitrite-mediated hepatic injury in rats was induced by administration of galactosamine/lipopolysaccharide (GalN/LPS). Twenty-four hours after GalN/LPS treatment, plasma ALT and AST levels δ increased significantly. However, pretreatment with 4G-α-D-glucopyranosyl rutin, a quercetin glycoside (30 mg/kg body weight), prevented these increases and reduced nitrotyrosine formation, indicating that consumption of quercetin glycosides prevent oxidative hepatotoxicity. Moreover, physiological levels of Q3′S and Q3GA (1 µM) effectively prevented peroxynitrite-induced nitrotyrosine formation in human serum albumin in in vitro experiments. These findings indicate peroxynitrite-induced oxidative hepatotoxicity is protected by the in vivo metabolites of quercetin, Q3′S and Q3GA.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Crozier, Professor Alan
Authors: Yokoyama, A., Sakakibara, H., Crozier, A., Kawai, Y., Matsui, A., Terao, J., Kumazawa, S., and Shimoi, K.
Subjects:Q Science > QH Natural history > QH301 Biology
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Free Radical Research
ISSN:1071-5762
ISSN (Online):1029-2470

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