Isolation, characterization, and differentiation of Thy1.1-sorted pancreatic adult progenitor cell populations

Stevenson, K.S., McGlynn, L.M., Hodge, M., McLinden, H., George, W.D., Davies, R.W. and Shiels, P.G. (2009) Isolation, characterization, and differentiation of Thy1.1-sorted pancreatic adult progenitor cell populations. Stem Cells and Development, 18(10), pp. 1389-1398. (doi:10.1089/scd.2008.0301)

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Publisher's URL: http://dx.doi.org/10.1089/scd.2008.0301

Abstract

We have isolated a novel progenitor cell population from adult rat pancreatic ducts, termed pancreatic-derived progenitor cells (PDPCs). Here, we report the <i>in vitro</i> culture, selection, and characterization of Thy1.1-positive and Thy1.1-negative PDPC subpopulations. These cells exhibit bipotentiality for differentiation into both pancreatic and hepatic cell types. Significantly, they express Pdx-1. Using a serum-free FGF-4-containing differentiation protocol, we have observed a time course of both morphological and gene expression changes indicative of hepatic lineage differentiation for the Thy1.1-positive subpopulation. These cells express albumin and store glycogen, typical features of mature hepatocytes. The Thy1.1-positive subpopulation could also readily be induced to differentiate into a pancreatic lineage with characteristic morphological changes resulting in three-dimensional islet-like structures and the transcriptional expression of insulin and glucagon in addition to Pdx-1. No morphological evidence of islet-like clusters was observed using the Thy1.1-negative population. However, Thy1.1-negative cells grown in pancreatic differentiation medium did show insulin gene transcription. Glucagon was not expressed in the undifferentiated Thy1.1-negative cells, nor was it induced in vitro after differentiation. The detection of Pdx-1 transcriptional expression in both populations indicates their potential as a novel source of non-β-cell-derived insulin.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shiels, Professor Paul and McGlynn, Dr Liane and Davies, Professor Roger
Authors: Stevenson, K.S., McGlynn, L.M., Hodge, M., McLinden, H., George, W.D., Davies, R.W., and Shiels, P.G.
Subjects:Q Science > QH Natural history > QH301 Biology
R Medicine > R Medicine (General)
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Stem Cells and Development
Publisher:Mary Ann Liebert, Inc. Publishers
ISSN:1547-3287
ISSN (Online):1557-8534
Published Online:27 March 2009
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