Dynamic regulation of guard cell anion channels by cytosolic-free Ca2+ concentration and protein phosphorylation

Chen, Z.-H., Hills, A., Lim, C.K. and Blatt, M.R. (2010) Dynamic regulation of guard cell anion channels by cytosolic-free Ca2+ concentration and protein phosphorylation. Plant Journal, 61(5), pp. 816-825. (doi:10.1111/j.1365-313X.2009.04108.x)

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Abstract

In guard cells, activation of anion channels (I(anion)) is an early event leading to stomatal closure. Activation of I(anion) has been associated with abscisic acid (ABA) and its elevation of cytosolic free Ca2+ concentration ([Ca2+](i)). However, the dynamics of [Ca2+](i) action on I(anion) have never established, despite their importance for understanding the mechanics of stomatal adaptation to stress. We have quantified the [Ca2+](i) dynamics of I(anion) in Vicia faba guard cells, measuring channel current under voltage clamp while manipulating and recording [Ca2+](i) using Fura-2 fluorescence imaging. We found I(anion) to rise with [Ca2+](i) only at concentrations substantially above the mean resting value of 125+/-13 nM, yielding an apparent K(d) of 720+/-65 nM and Hill coefficient consistent with the binding of 3-4 Ca2+ ions to activate the channels. Approximately 30% of guard cells exhibited a baseline of I(anion) activity, but without a [Ca2+](i) dependence to the current. The protein phosphatase antagonist okadaic acid increased this current baseline over 2-fold. Additionally, okadaic acid altered the [Ca2+](i)-sensitivity of I(anion), displacing the apparent K(d) for [Ca2+](i) to 573 +/- 38 nM. These findings support previous evidence for different modes of regulation for I(anion), only one of which depends on [Ca2+](i), and they underscore an independence of [Ca2+](i) from protein (de-)phosphorylation in controlling I(anion). Most important, our results demonstrate a significant displacement of I(anion) sensitivity to higher [Ca2+](i) compared with that of the guard cell K+ channels, implicating a capacity for variable dynamics between net osmotic solute uptake and loss.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hills, Mr Adrian and Blatt, Professor Michael and Chen, Dr Zhonghua
Authors: Chen, Z.-H., Hills, A., Lim, C.K., and Blatt, M.R.
Subjects:Q Science > QH Natural history > QH301 Biology
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Journal
ISSN:0960-7412
ISSN (Online):1365-313X
Published Online:15 December 2009

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
452562Analysis of membrane traffic in adaptive stress tolerance in plantsMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/F001630/1Institute of Molecular Cell and Systems Biology