Identification of metabolites in human plasma and urine after consumption of a polyphenol-rich juice drink

Mullen, W., Borges, G., Lean, M.E.J., Roberts, S.A. and Crozier, A. (2010) Identification of metabolites in human plasma and urine after consumption of a polyphenol-rich juice drink. Journal of Agricultural and Food Chemistry, 58(4), pp. 2586-2595. (doi:10.1021/jf904096v)

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Publisher's URL: http://dx.doi.org/10.1021/jf904096v

Abstract

A polyphenol-rich (P-R) juice drink was developed as a potential approach to increase intake of dietary polyphenols. Analysis of the beverage by HPLC with PDA, fluorescence, and MS detection facilitated the identification/partial identification of 40 flavonoids and related phenolic compounds. The main constituents were (GêÆ)-epigallocatechin and other green tea flavan-3-ols, phloretin-2GǦ-O-glucoside, gallic acid, hesperetin-7-O-rutinoside, 5-O-caffeoylquinic acid, and procyanidins, with trace levels of several flavonols and purple grape juice anthocyanins also being present. Healthy human subjects (n = 10) consumed 350 mL of the P-R juice drink, after which plasma and urine samples were collected over a 0GêÆ24 h period. HPLC-MS analysis identified 13 metabolites in plasma and a further 20 in urine. Qualitatively, the profiles of the glucuronide, sulfated, and methylated metabolites were very similar to those detected in earlier investigations when the main components in the juice drink were consumed separately in feeding studies with coffee, green tea, orange juice, and apple cider.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Borges, Dr Gina and Mullen, Dr Bill and Lean, Professor Michael and Crozier, Professor Alan
Authors: Mullen, W., Borges, G., Lean, M.E.J., Roberts, S.A., and Crozier, A.
Subjects:Q Science > QH Natural history > QH301 Biology
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of Agricultural and Food Chemistry
ISSN:0021-8561
ISSN (Online):1520-5118
Published Online:20 January 2010

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
467791Health Effects and Bioavailability of Antioxidants in a Beverage FormulationAlan CrozierThe Coca-Cola Company (COLA-COMP)Prof Crozier, AMVLS MED -HUMAN NUTRITION