Mullen, W. , Larcombe, S., Arnold, K., Welchman, H. and Crozier, A. (2010) Use of accurate mass full scan mass spectrometry for the analysis of anthocyanins in berries and berry-fed tissues. Journal of Agricultural and Food Chemistry, 58(7), pp. 3910-3915. (doi: 10.1021/jf902267v)
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Publisher's URL: http://dx.doi.org/10.1021/jf902267v
Abstract
Anthocyanins in extracts from raspberries and blueberries were analyzed by reversed-phase HPLC coupled to a high-resolution Exactive Orbitrap mass spectrometer (HR-MS) with a resolution of 100,000, operated with an electrospray source in the positive ionization mode. As consumption of anthocyanin-rich berry extracts has been associated with improved cognitive function, brain extracts from European greenfinches (Carduelis chloris) that had been fed one blackberry daily for a period of 2 weeks were analyzed by both HPLC with traditional tandem MS in the selected reaction monitoring mode and HPLC-HR-MS. Cyanidin-3-O-glucoside was detected in the brain extracts by both methods, but because of its high level of selectivity, HR-MS was ca. 200-fold more sensitive. A further advantage of HR-MS is that unlike MS-SRM it enables both targeted and nontargeted compounds to be detected and much lower limits of detection are achieved without compromising the selectivity of the analysis
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Mullen, Dr Bill and Arnold, Dr Kathryn and Crozier, Professor Alan and Larcombe, Dr Stephen |
Authors: | Mullen, W., Larcombe, S., Arnold, K., Welchman, H., and Crozier, A. |
Subjects: | Q Science > QH Natural history |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Journal of Agricultural and Food Chemistry |
ISSN: | 0021-8561 |
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