BAF45b Is required for efficient Zika virus infection of HAP1 cells

Persson, B. D., Nord, S., Lindqvist, R., Danskog, K., Överby, A. K., Kohl, A. , Willison, H. J. , Lenman, A. and Arnberg, N. (2021) BAF45b Is required for efficient Zika virus infection of HAP1 cells. Viruses, 13(10), 2007. (doi: 10.3390/v13102007) (PMCID:PMC8540262)

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Abstract

The 2016 Zika virus (ZIKV) epidemic illustrates the impact of flaviviruses as emerging human pathogens. For unknown reasons, ZIKV replicates more efficiently in neural progenitor cells (NPCs) than in postmitotic neurons. Here, we identified host factors used by ZIKV using the NCI-60 library of cell lines and COMPARE analysis, and cross-analyzed this library with two other libraries of host factors with importance for ZIKV infection. We identified BAF45b, a subunit of the BAF (Brg1/Brm-associated factors) protein complexes that regulate differentiation of NPCs to post-mitotic neurons. ZIKV (and other flaviviruses) infected HAP1 cells deficient in expression of BAF45b and other BAF subunits less efficiently than wildtype (WT) HAP1 cells. We concluded that subunits of the BAF complex are important for infection of ZIKV and other flavivirus. Given their function in cell and tissue differentiation, such regulators may be important determinants of tropism and pathogenesis of arthropod-borne flaviviruses.

Item Type:Articles
Additional Information:Funding: This research was funded by ZikaPLAN, European Union’s Horizon 2020 research and innovation programme under grant agreement no. 734584 (N.A.), the Swedish Research Council grant no. 2017-02438 (A.Ö.), and the UK Medical Research Council grants no. MC_UU_12014/8 and no. MR/N017552/1 (A.K.). We also acknowledge the Biochemical Imaging Center at Umeå university and the National Microscopy Infrastructure, NMI (VR-RFI 2016-00968) for assisting with microscopy.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Willison, Professor Hugh and Kohl, Professor Alain
Authors: Persson, B. D., Nord, S., Lindqvist, R., Danskog, K., Överby, A. K., Kohl, A., Willison, H. J., Lenman, A., and Arnberg, N.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Viruses
Publisher:MDPI
ISSN:1999-4915
ISSN (Online):1999-4915
Published Online:06 October 2021
Copyright Holders:Copyright © 2021 by the authors.
First Published:First published in Viruses 13(10):2007
Publisher Policy:Reproduced under a Creative Commons Licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656551Arbovirus interactions with arthropod hostsAlain KohlMedical Research Council (MRC)MC_UU_12014/8MVLS III - CENTRE FOR VIRUS RESEARCH
172580The emergence of Zika virus in Brazil: investigating prevalence and host responses to design preventive strategiesAlain KohlMedical Research Council (MRC)MR/N017552/1III-MRC-GU Centre for Virus Research