The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease

Wright, P. B. et al. (2021) The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease. Scientific Reports, 11, 19616. (doi: 10.1038/s41598-021-98611-7) (PMID:34608184) (PMCID:PMC8490356)

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Abstract

To understand the contribution of mononuclear phagocytes (MNP), which include monocyte-derived intestinal macrophages, to the pathogenesis of inflammatory bowel disease (IBD), it is necessary to identify functionally-different MNP populations. We aimed to characterise intestinal macrophage populations in patients with IBD. We developed 12-parameter flow cytometry protocols to identify and human intestinal MNPs. We used these protocols to purify and characterize colonic macrophages from colonic tissue from patients with Crohn’s disease (CD), ulcerative colitis (UC), or non-inflamed controls, in a cross-sectional study. We identify macrophage populations (CD45+CD64+ HLA-DR+) and describe two distinct subsets, differentiated by their expression of the mannose receptor, CD206. CD206+ macrophages expressed markers consistent with a mature phenotype: high levels of CD68 and CD163, higher transcription of IL-10 and lower expression of TREM1. CD206− macrophages appear to be less mature, with features more similar to their monocytic precursors. We identified and purified macrophage populations from human colon. These appear to be derived from a monocytic precursor with high CCR2 and low CD206 expression. As these cells mature, they acquire expression of IL-10, CD206, CD63, and CD168. Targeting the newly recruited monocyte-derived cells may represent a fruitful avenue to ameliorate chronic inflammation in IBD.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Clay, Slater and Heawood, Miss Anna and Robertson, Dr Elaine and McDonald, Miss Elizabeth and Wright, Miss Pamela and Bravo-Blas, Dr Alberto and Bain, Mr Calum and Morton, Mr Fraser and Milling, Professor Simon and Baer, Hannah and Gaya, Mr Daniel and Ijaz, Dr Umer and Mowat, Professor Allan and Nijjar, Dr Jagtar
Authors: Wright, P. B., McDonald, E., Bravo-Blas, A., Baer, H., Heawood, A., Bain, C. C., Mowat, A. M., Clay, S. L., Robertson, E. V., Morton, F., Nijjar, J. S., Ijaz, U. Z., Milling, S. W.F., and Gaya, D. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Science and Engineering > School of Engineering > Infrastructure and Environment
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Scientific Reports 11: 19616
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
168458Comparing the immunophenotype of common inflammatory diseases to understand their pathogenesis.Simon MillingOffice of the Chief Scientific Adviser (CSO)ETM/304III - Immunology