SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles

Aithal, S., Mishriki, S., Gupta, R. , Sahu, R. P., Botos, G., Tanvir, S., Hanson, R. W. and Puri, I. K. (2022) SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles. Talanta, 236, 122841. (doi: 10.1016/j.talanta.2021.122841) (PMID:34635231) (PMCID:PMC8409056)

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Abstract

A rapid detection test for SARS-CoV-2 is urgently required to monitor virus spread and containment. Here, we describe a test that uses nanoprobes, which are gold nanoparticles functionalized with an aptamer specific to the spike membrane protein of SARS-CoV-2. An enzyme-linked immunosorbent assay confirms aptamer binding with the spike protein on gold surfaces. Protein recognition occurs by adding a coagulant, where nanoprobes with no bound protein agglomerate while those with sufficient bound protein do not. Using plasmon absorbance spectra, the nanoprobes detect 16 nM and higher concentrations of spike protein in phosphate-buffered saline. The time-varying light absorbance is examined at 540 nm to determine the critical coagulant concentration required to agglomerates the nanoprobes, which depends on the protein concentration. This approach detects 3540 genome copies/μl of inactivated SARS-CoV-2.

Item Type:Articles
Additional Information:This work was supported by the MITACS Accelerate Fund (IT18550) and Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant (RGPIN-2019-06571).
Keywords:SARS-CoV-2, spike protein, aptamer, biosensing, surface plasmon resonance, gold nanoparticle.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gupta, Dr Rohit
Creator Roles:
Gupta, R.Methodology, Investigation, Writing – review and editing
Authors: Aithal, S., Mishriki, S., Gupta, R., Sahu, R. P., Botos, G., Tanvir, S., Hanson, R. W., and Puri, I. K.
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:Talanta
Publisher:Elsevier
ISSN:0039-9140
ISSN (Online):1873-3573
Published Online:01 September 2021
Copyright Holders:Copyright © 2021 Elsevier
First Published:First published in Talanta 236:122841
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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