Validation of a point-of-care circulating cathodic antigen urine cassette test for Schistosoma mansoni diagnosis in the Sahel, and potential cross-reaction in pregnancy

Greter, H., Krauth, S. J. , Ngandolo, B. N. R., Alfaroukh, I. O., Zinsstag, J. and Utzinger, J. (2016) Validation of a point-of-care circulating cathodic antigen urine cassette test for Schistosoma mansoni diagnosis in the Sahel, and potential cross-reaction in pregnancy. American Journal of Tropical Medicine and Hygiene, 94(2), pp. 361-364. (doi: 10.4269/ajtmh.15-0577) (PMID:26556831) (PMCID:PMC4751945)

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Abstract

On the shores of Lake Chad, schistosomiasis among mobile pastoralists was investigated in a field laboratory. Point-of-care circulating cathodic antigen (POC-CCA) cassette test, reagent strip, and filtration were conducted on urine samples. Fresh stool samples were subjected to the Kato-Katz technique, and fixed samples were examined with an ether-concentration method at a reference laboratory. POC-CCA urine cassette tests revealed a Schistosoma mansoni prevalence of 6.9%, compared with only 0.5% by stool microscopy. Three pregnant women with otherwise negative urine and stool testing had positive POC-CCA. This observation raises concern of cross-reactivity in pregnancy. Hence, two pregnant women in Switzerland with no history of schistosomiasis were subjected to POC-CCA and one tested positive. Our data suggest that POC-CCA can be performed under extreme Sahelian conditions (e.g., temperatures > 40°C), and it is more sensitive than stool microscopy for S. mansoni diagnosis. However, potential cross-reactivity in pregnancy needs further investigation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Krauth, Dr Stefanie
Authors: Greter, H., Krauth, S. J., Ngandolo, B. N. R., Alfaroukh, I. O., Zinsstag, J., and Utzinger, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:American Journal of Tropical Medicine and Hygiene
Publisher:American Society of Tropical Medicine and Hygiene
ISSN:0002-9637
ISSN (Online):1476-1645
Published Online:10 November 2015

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