Depletion of a Toxoplasma porin leads to defects in mitochondrial morphology and contacts with the ER

Mallo, N. et al. (2021) Depletion of a Toxoplasma porin leads to defects in mitochondrial morphology and contacts with the ER. Journal of Cell Science, 134(20), jcs255299. (doi: 10.1242/jcs.255299) (PMID:34523684)

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Abstract

The Voltage Dependent Anion channel (VDAC) is a ubiquitous channel in the outer membrane of the mitochondrion with multiple roles in protein, metabolite and small molecule transport. In mammalian cells, VDAC, as part of a larger complex including the inositol triphosphate receptor, has been shown to have a role in mediating contacts between the mitochondria and endoplasmic reticulum (ER). We identify VDAC of the pathogenic apicomplexan Toxoplasma gondii and demonstrate its importance for parasite growth. We show that VDAC is involved in protein import and metabolite transfer to mitochondria. Further, depletion of VDAC resulted in significant morphological changes of the mitochondrion and ER, suggesting a role in mediating contacts between these organelles in T. gondii.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Biddau, Dr Marco and Harding, Dr Clare and Ovciarikova, Miss Jana and Sheiner, Dr Lilach and Lemgruber Soares, Dr Leandro and Mallo, Dr Natalia
Authors: Mallo, N., Ovciarikova, J., Martins-Duarte, E. S., Baehr, S. C., Biddau, M., Wilde, M.-L., Uboldi, A. D., Lemgruber, L., Tonkin, C. J., Wideman, J. G., Harding, C. R., and Sheiner, L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Cell Science
Publisher:Company of Biologists
ISSN:0021-9533
ISSN (Online):1477-9137
Published Online:15 September 2021
Copyright Holders:Copyright © 2021 The Company of Biologists Ltd
First Published:First published in Journal of Cell Science 134(20): jcs255299
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172187Dissection of the unusual mitochondrial tRNA import translocon of Toxoplasma gondiiLilach SheinerBiotechnology and Biological Sciences Research Council (BBSRC)BB/N003675/1Institute of Infection, Immunity & Inflammation
307056Lost in translation. A study of the divergent mitochondrial translation pathway of the parasite Toxoplasma gondiiLilach SheinerWellcome Trust (WELLCOTR)217173/Z/19/ZMVLS - Polyomics Facility
304336Iron usage in the eukaryotic parasite Toxoplasma gondiiClare HardingWellcome Trust (WELLCOTR)213455/Z/18/ZIII - Parasitology