Dos-Santos, J. C. K. et al. (2020) Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. PLoS Neglected Tropical Diseases, 14(7), e0007656. (doi: 10.1371/journal.pntd.0007656) (PMID:32687542) (PMCID:PMC7392343)
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Abstract
Platelets drive endothelial cell activation in many diseases. However, if this occurs in Plasmodium vivax malaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterations during acute illness. We performed platelet flow cytometry of PAC-1 and P-selectin. We measured platelet markers (CXCL4, CD40L, P-selectin, Thrombopoietin, IL-11) and endothelial activation markers (ICAM-1, von Willebrand Factor and E-selectin) in plasma with a multiplex-based assay. The values of each mediator were used to generate heatmaps, K-means clustering and Principal Component analysis. In addition, we determined pair-wise Pearson’s correlation coefficients to generate correlation networks. Platelet counts were reduced, and mean platelet volume increased in malaria patients. The activation of circulating platelets in flow cytometry did not differ between patients and controls. CD40L levels (Median [IQ]: 517 [406–651] vs. 1029 [732–1267] pg/mL, P = 0.0001) were significantly higher in patients, while P-selectin and CXCL4 showed a nonsignificant trend towards higher levels in patients. The network correlation approach demonstrated the correlation between markers of platelet and endothelial activation, and the heatmaps revealed a distinct pattern of activation in two subsets of P. vivax patients when compared to controls. Although absolute platelet activation was not strong in uncomplicated vivax malaria, markers of platelet activity and production were correlated with higher endothelial cell activation, especially in a specific subset of patients.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Da Silva Filho, Dr Joao |
Creator Roles: | Silva-Filho, J. L.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft |
Authors: | Dos-Santos, J. C. K., Silva-Filho, J. L., Judice, C. C., Kayano, A. C. A. V., Aliberti, J., Khouri, R., de Lima, D. S., Nakaya, H., Lacerda, M. V. G., De Paula, E. V., Lopes, S. C. P., and Costa, F. T. M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | PLoS Neglected Tropical Diseases |
Publisher: | Public Library of Science |
ISSN: | 1935-2727 |
ISSN (Online): | 1935-2735 |
Published Online: | 20 July 2020 |
Copyright Holders: | This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose |
First Published: | First published in PLoS Neglected Tropical Diseases 14(7): e0007656 |
Publisher Policy: | Reproduced under a Creative Commons License |
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