Tuft cells increase following ovine intestinal parasite infections and define evolutionarily conserved and divergent responses

Hildersley, K. A., Mcneilly, T. N., Gillan, V. , Otto, T. D. , Löser, S., Gerbe, F., Jay, P., Maizels, R. M. , Devaney, E. and Britton, C. (2021) Tuft cells increase following ovine intestinal parasite infections and define evolutionarily conserved and divergent responses. Frontiers in Immunology, 12, 781108. (doi: 10.3389/fimmu.2021.781108) (PMID:34880874) (PMCID:PMC8646091)

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Abstract

Helminth parasite infections of humans and livestock are a global health and economic problem. Resistance of helminths to current drug treatment is an increasing problem and alternative control approaches, including vaccines, are needed. Effective vaccine design requires knowledge of host immune mechanisms and how these are stimulated. Mouse models of helminth infection indicate that tuft cells, an unusual type of epithelial cell, may ‘sense’ infection in the small intestine and trigger a type 2 immune response. Currently nothing is known of tuft cells in immunity in other host species and in other compartments of the gastrointestinal (GI) tract. Here we address this gap and use immunohistochemistry and single cell RNA-sequencing to detail the presence and gene expression profile of tuft cells in sheep following nematode infections. We identify and characterize tuft cells in the ovine abomasum (true stomach of ruminants) and show that they increase significantly in number following infection with the globally important nematodes Teladorsagia circumcincta and Haemonchus contortus. Ovine abomasal tuft cells show enriched expression of tuft cell markers POU2F3, GFI1B, TRPM5 and genes involved in signaling and inflammatory pathways. However succinate receptor SUCNR1 and free fatty acid receptor FFAR3, proposed as ‘sensing’ receptors in murine tuft cells, are not expressed, and instead ovine tuft cells are enriched for taste receptor TAS2R16 and mechanosensory receptor ADGRG6. We also identify tuft cell sub-clusters at potentially different stages of maturation, suggesting a dynamic process not apparent from mouse models of infection. Our findings reveal a tuft cell response to economically important parasite infections and show that while tuft cell effector functions have been retained during mammalian evolution, receptor specificity has diverged. Our data advance knowledge of host-parasite interactions in the GI mucosa and identify receptors that may potentiate type 2 immunity for optimized control of parasitic nematodes.

Item Type:Articles
Additional Information:Funding KH is supported by an Industrial Partnership PhD studentship funded by University of Glasgow, Moredun Foundation and Pentlands Science Park, UK. TM is supported by the Scottish Government’s Rural Affairs, Food and the Environment (RAFE) Strategic Research Portfolio 2016-2021. VG and SL are funded by a Wellcome Trust Collaborative Award (Ref 211814) to CB, ED, TM, PJ, and RM; SL and RM also received Wellcome Trust support through an Investigator Grant (Ref 219530), and the Wellcome Trust core-funded Wellcome Centre for Integrative Parasitology (Ref 104111). TDO is supported by Wellcome Trust grant 104111/Z/14/ZR.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Devaney, Professor Eileen and Mcneilly, Dr Tom and Britton, Dr Collette and Otto, Dr Thomas and Loeser, Dr Stephan and Gillan, Dr Victoria and Hildersley, Katie and Maizels, Professor Rick
Authors: Hildersley, K. A., Mcneilly, T. N., Gillan, V., Otto, T. D., Löser, S., Gerbe, F., Jay, P., Maizels, R. M., Devaney, E., and Britton, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Frontiers in Immunology
Publisher:Frontiers Media
ISSN:1664-3224
ISSN (Online):1664-3224
Copyright Holders:Copyright © 2021 Hildersley, McNeilly, Gillan, Otto, Löser, Gerbe, Jay, Maizels, Devaney and Britton.
First Published:First published in Frontiers in Immunology 12:781108
Publisher Policy:Reproduced under a Creative Commons Licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
170547The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/ZRIII - Parasitology
302416Tuft cells, sensors adn effectors in immunity to helminthsRichard MaizelsWellcome Trust (WELLCOTR)211814/Z/18/ZIII - Parasitology
308411Molecular and Cellular Interactions in Helminth InfectionsRichard MaizelsWellcome Trust (WELLCOTR)219530/Z/19/ZIII - Parasitology