Engineering of the membrane of fibroblast cells with virus-specific antibodies: a novel biosensor tool for virus detection

Moschopoulou, G. et al. (2008) Engineering of the membrane of fibroblast cells with virus-specific antibodies: a novel biosensor tool for virus detection. Biosensors and Bioelectronics, 24(4), pp. 1027-1030. (doi: 10.1016/j.bios.2008.06.039) (PMID:18693110)

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Abstract

A novel concept for the assay of viral antigens is described. The methodological approach is based on a membrane-engineering process involving the electroinsertion of virus-specific antibodies in the membranes of fibroblast cells. As a representative example, Vero fibroblasts were engineered with antibodies against Cucumber mosaic virus (CMV) and used for the construction of an ultra-sensitive miniature cell biosensor system. The attachment of a homologous virus triggered specific changes to the cell membrane potential that were measured by appropriate microelectrodes, according to the principle of the bioelectric recognition assay (BERA). No change in the membrane potential was observed upon cell contact with the heterologous cucumber green mottle mosaic virus (CGMMV). Fluorescence microscopy observations showed that attachment of CMV particles to membrane-engineered cells was associated with membrane hyperpolarization and increased [Ca2+]cyt. In an additional field-based application, we were able to detect CMV-infected tobacco plants at an essentially 100% level of accuracy.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nomikou, Dr Kyriaki
Authors: Moschopoulou, G., Vitsa, K., Bem, F., Vassilakos, N., Perdikaris, A., Blouhos, P., Yialouris, C., Frosyniotis, D., Anthopoulos, I., Mangana, O., Nomikou, K., Rodeva, V., Kostova, D., Grozeva, S., Michaelides, A., Simonian, A., and Kintzios, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Biosensors and Bioelectronics
Publisher:Elsevier
ISSN:0956-5663
ISSN (Online):1873-4235
Published Online:04 July 2008

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