Group V secretory phospholipase A2 is involved in tubular integrity and sodium handling in the kidney

Silva-Filho, J. L. et al. (2016) Group V secretory phospholipase A2 is involved in tubular integrity and sodium handling in the kidney. PLoS ONE, 11(1), e0147785. (doi: 10.1371/journal.pone.0147785) (PMID:26820468) (PMCID:PMC4731149)

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Abstract

Group V (GV) phospholipase A2 (PLA2) is a member of the family of secreted PLA2 (sPLA2) enzymes. This enzyme has been identified in several organs, including the kidney. However, the physiologic role of GV sPLA2 in the maintenance of renal function remains unclear. We used mice lacking the gene encoding GV sPLA2 (Pla2g5−/−) and wild-type breeding pairs in the experiments. Mice were individually housed in metabolic cages and 48-h urine was collected for biochemical assays. Kidney samples were evaluated for glomerular morphology, renal fibrosis, and expression/activity of the (Na+ + K+)-ATPase α1 subunit. We observed that plasma creatinine levels were increased in Pla2g5−/− mice following by a decrease in creatinine clearance. The levels of urinary protein were higher in Pla2g5−/− mice than in the control group. Markers of tubular integrity and function such as γ-glutamyl transpeptidase, lactate dehydrogenase, and sodium excretion fraction (FENa+) were also increased in Pla2g5−/− mice. The increased FENa+ observed in Pla2g5−/− mice was correlated to alterations in cortical (Na+ + K+) ATPase activity/ expression. In addition, the kidney from Pla2g5−/− mice showed accumulation of matrix in corticomedullary glomeruli and tubulointerstitial fibrosis. These data suggest GV sPLA2 is involved in the maintenance of tubular cell function and integrity, promoting sodium retention through increased cortical (Na+ + K+)-ATPase expression and activity.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Da Silva Filho, Dr Joao
Authors: Silva-Filho, J. L., Peruchetti, D. B., Moraes-Santos, F., Landgraf, S. S., Silva, L. S., Sirtoli, G. M., Zamith-Miranda, D., Takiya, C. M., Pinheiro, A. A. S., Diaz, B. L., and Caruso-Neves, C.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2016 Silva-Filho et al.
First Published:First published in PLoS ONE 11(1): e0147785
Publisher Policy:Reproduced under a Creative Commons License

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