Sheep poxvirus identification by PCR in cell cultures

Mangana-Vougiouka, O., Markoulatos, P., Koptopoulos, G., Nomikou, K. , Bakandritsos, N. and Papadopoulos, O. (1999) Sheep poxvirus identification by PCR in cell cultures. Journal of Virological Methods, 77(1), pp. 75-79. (doi: 10.1016/S0166-0934(98)00138-4) (PMID:10029327)

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Abstract

A simple, rapid and specific diagnostic polymerase chain reaction (PCR) method was developed for sheep poxvirus identification. The primers used were from the sequenced genomes of the capripox viruses KS-1 and InS-1. Six different sheep pox isolates were tested against two orf (parapox) and three animal herpesviruses as controls. Material from uninfected cell cultures was also used as control. The sensitivity of the PCR was approximately equivalent with each of the two primers and for the six sheep pox isolates. All the negative control virus DNAs were negative and differed clearly from those of the sheep pox strains.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nomikou, Dr Kyriaki
Authors: Mangana-Vougiouka, O., Markoulatos, P., Koptopoulos, G., Nomikou, K., Bakandritsos, N., and Papadopoulos, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virological Methods
Publisher:Elsevier
ISSN:0166-0934
ISSN (Online):1879-0984
Published Online:06 January 1999

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