Detection of sheep poxvirus in skin biopsy samples by a multiplex polymerase chain reaction

Markoulatos, P., Mangana-Vougiouka, O., Koptopoulos, G., Nomikou, K. and Papadopoulos, O. (2000) Detection of sheep poxvirus in skin biopsy samples by a multiplex polymerase chain reaction. Journal of Virological Methods, 84(2), pp. 161-167. (doi: 10.1016/S0166-0934(99)00141-X) (PMID:10680965)

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Abstract

The development of a multiplex polymerase chain reaction (PCR) method with amplification of capripoxvirus in a single-step procedure from skin biopsies using three primer pairs, two specific for capripoxvirus and one specific for α-tubulin is described. A sensitive multiplex PCR was achieved by optimization of parameters such as the primer concentrations, magnesium and dNTPs concentrations. False negative results that sometimes arise due to inhibitors of DNA amplification may be avoided by the inclusion in the assay of α-tubulin primers. The results reported on 42 skin biopsies from sheep suspected to have poxvirus infection, indicated that the assay could monitor simultaneously DNA extraction from skin biopsy samples and allow improved detection of capripoxvirus within 24 h of specimen receipt in the laboratory.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nomikou, Dr Kyriaki
Authors: Markoulatos, P., Mangana-Vougiouka, O., Koptopoulos, G., Nomikou, K., and Papadopoulos, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virological Methods
Publisher:Elsevier
ISSN:0166-0934
ISSN (Online):1879-0984
Published Online:24 January 2000

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