RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication

Anthony, S.J., Darpel, K.E., Belaganahalli, M.N., Maan, N., Nomikou, K. , Sutton, G., Attoui, H., Maan, S. and Mertens, P.P.C. (2011) RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication. Virology, 420(2), pp. 164-171. (doi: 10.1016/j.virol.2011.09.009) (PMID:21968198)

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Abstract

This paper reports a concatemeric RNA in a strain of epizootic haemorrhagic disease virus (EHDV) serotype 5. Sequencing showed that the concatemeric RNA contains two identical full-length copies of genome segment 9, arranged in series, which has apparently replaced the monomeric form of the segment. In vitro translation demonstrated that the concatemeric RNA can act as a viable template for VP6 translation, but that no double-sized protein is produced. Studies were also performed to assess whether mutations might be easily introduced into the second copy (which might indicate some potential evolutionary significance of a concatemeric RNA segment), however multiple (n = 40) passages generated no changes in the sequence of either the upstream or downstream segments. Further, we present results that demonstrate the presence of concatemers or partial gene duplications in multiple segments of different orbiviruses (in tissue culture and purified virus), suggesting their generation is likely to be a normal feature of orbivirus replication.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nomikou, Dr Kyriaki
Authors: Anthony, S.J., Darpel, K.E., Belaganahalli, M.N., Maan, N., Nomikou, K., Sutton, G., Attoui, H., Maan, S., and Mertens, P.P.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
Publisher:Elsevier
ISSN:0042-6822
ISSN (Online):1096-0341
Published Online:02 October 2011

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