SWCNTs/PEDOT:PSS-modified microelectrode arrays for dual-mode detection of electrophysiological signals and dopamine concentration in the striatum under isoflurane anesthesia

He, E. et al. (2021) SWCNTs/PEDOT:PSS-modified microelectrode arrays for dual-mode detection of electrophysiological signals and dopamine concentration in the striatum under isoflurane anesthesia. ACS Sensors, 6(9), pp. 3377-3386. (doi: 10.1021/acssensors.1c01241) (PMID:34410704)

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Abstract

Accurate detection of the degree of isoflurane anesthesia during a surgery is important to avoid the risk of overdose isoflurane anesthesia timely. To address this challenge, a four-shank implantable microelectrode array (MEA) was fabricated for the synchronous real-time detection of dual-mode signals [electrophysiological signal and dopamine (DA) concentration] in rat striatum. The SWCNTs/PEDOT:PSS nanocomposites were modified onto the MEAs, which significantly improved the electrical and electrochemical performances of the MEAs. The electrical performance of the modified MEAs with a low impedance (16.20 ± 1.68 kΩ) and a small phase delay (-27.76 ± 0.82°) enabled the MEAs to detect spike firing with a high signal-to-noise ratio (> 3). The electrochemical performance of the modified MEAs with a low oxidation potential (160 mV), a low detection limit (10 nM), high sensitivity (217 pA/μM), and a wide linear range (10 nM-72 μM) met the specific requirements for DA detection in vivo. The anesthetic effect of isoflurane was mediated by inhibiting the spike firing of D2_SPNs (spiny projection neurons expressing the D2-type DA receptor) and the broadband oscillation rhythm of the local field potential (LFP). Therefore, the spike firing rate of D2_SPNs and the power of LFP could reflect the degree of isoflurane anesthesia together. During the isoflurane anesthesia-induced death procedure, we found that electrophysiological activities and DA release were strongly inhibited, and changes in the DA concentration provided more details regarding this procedure. The dual-mode recording MEA provided a detection method for the degree of isoflurane anesthesia and a prediction method for fatal overdose isoflurane anesthesia.

Item Type:Articles
Additional Information:This work was sponsored by the National Natural Science Foundation of China (Nos. 61960206012, 62121003, 61971400, 61771452 61775216, 61975206, and 61973292), the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No. GJJSTD20210004), the National Key Research and Development Program (2017YFA0205902), the Youth Innovation Promotion Association, CAS, and the opening foundation of the State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center (No. SMFA19K05).
Keywords:PEDOT, electrophysiological signal, dopamine, isoflurane anesthesia, SWCNT, striatum, microelectrode array.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing
Authors: He, E., Xu, S., Dai, Y., Wang, Y., Xiao, G., Xie, J., Xu, S., Fan, P., Mo, F., Wang, M., Song, Y., Yin, H., Li, Y., Wang, Y., and Cai, X.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Sensors
Publisher:American Chemical Society
ISSN:2379-3694
ISSN (Online):2379-3694
Published Online:19 August 2021
Copyright Holders:Copyright © 2021 American Chemical Society
First Published:First published in ACS Sensors 6(9): 3377-3386
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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