Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding

Sullivan, S. , Waksman, T., Paliogianni, D., Henderson, L., Lütkemeyer, M., Suetsugu, N. and Christie, J. M. (2021) Regulation of plant phototropic growth by NPH3/RPT2-like substrate phosphorylation and 14-3-3 binding. Nature Communications, 12, 6129. (doi: 10.1038/s41467-021-26333-5) (PMID:34675214) (PMCID:PMC8531357)

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Abstract

Polarity underlies all directional growth responses in plants including growth towards the light (phototropism). The plasma-membrane associated protein, NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key determinant of phototropic growth which is regulated by phototropin (phot) AGC kinases. Here we demonstrate that NPH3 is directly phosphorylated by phot1 within a conserved C-terminal consensus sequence (RxS) that is necessary to promote phototropism and petiole positioning in Arabidopsis. RxS phosphorylation also triggers 14-3-3 binding combined with changes in NPH3 phosphorylation and localisation status. Mutants of NPH3 that are unable to bind or constitutively bind 14-3-3 s show compromised functionality consistent with a model where phototropic curvature is established by signalling outputs arising from a gradient of NPH3 RxS phosphorylation across the stem. Our findings therefore establish that NPH3/RPT2-Like (NRL) proteins are phosphorylation targets for plant AGC kinases. Moreover, RxS phosphorylation is conserved in other members of the NRL family, suggesting a common mechanism of regulating plant growth to the prevailing light environment.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Suetsugu, Dr Noriyuki and Lutkemeyer, Ms Melanie Katja and Christie, Professor John and Henderson, Ms Louise and Sullivan, Dr Stuart and Paliogianni, Dimitra and Waksman, Thomas
Authors: Sullivan, S., Waksman, T., Paliogianni, D., Henderson, L., Lütkemeyer, M., Suetsugu, N., and Christie, J. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Nature Communications
Publisher:Nature Research
ISSN:2041-1723
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Nature Communications 12: 6129
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
170684Photoreceptor Engineering to Modulate Plant GrowthJohn ChristieBiotechnology and Biological Sciences Research Council (BBSRC)BB/M002128/1MCSB - Plant Sciences
301413How do Phototropin Receptor Kinases Initiate Signalling from the PlasmaJohn ChristieBiotechnology and Biological Sciences Research Council (BBSRC)BB/R001499/1Institute of Molecular, Cell & Systems Biology