Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells

Carter, S. D. et al. (2020) Ribosome-associated vesicles: a dynamic subcompartment of the endoplasmic reticulum in secretory cells. Science Advances, 6(14), eaay9572. (doi: 10.1126/sciadv.aay9572) (PMID:32270040) (PMCID:PMC7112762)

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Abstract

The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo–electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Carter, Dr Stephen
Authors: Carter, S. D., Hampton, C. M., Langlois, R., Melero, R., Farino, Z. J., Calderon, M. J., Li, W., Wallace, C. T., Tran, N. H., Grassucci, R. A., Siegmund, S. E., Pemberton, J., Morgenstern, T. J., Eisenman, L., Aguilar, J. I., Greenberg, N. L., Levy, E. S., Yi, E., Mitchell, W. G., Rice, W. J., Wigge, C., Pilli, J., George, E. W., Aslanoglou, D., Courel, M., Freyberg, R. J., Javitch, J. A., Wills, Z. P., Area-Gomez, E., Shiva, S., Bartolini, F., Volchuk, A., Murray, S. A., Aridor, M., Fish, K. N., Walter, P., Balla, T., Fass, D., Wolf, S. G., Watkins, S. C., Carazo, J. M., Jensen, G. J., Frank, J., and Freyberg, Z.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Science Advances
Publisher:American Association for the Advancement of Science
ISSN:2375-2548
ISSN (Online):2375-2548
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Science Advances 6(14): eaay9572
Publisher Policy:Reproduced under a Creative Commons License

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