Carter, S.D. , Barr, J.N. and Edwards, T.A. (2012) Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein. Acta Crystallographica. Section F: Structural Biology Communications, 68(5), pp. 569-573. (doi: 10.1107/s1744309112009736) (PMID:22691790) (PMCID:PMC3374515)
Full text not currently available from Enlighten.
Abstract
Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, [beta] = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Carter, Dr Stephen |
Authors: | Carter, S.D., Barr, J.N., and Edwards, T.A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Acta Crystallographica. Section F: Structural Biology Communications |
Publisher: | International Union of Crystallography |
ISSN: | 1744-3091 |
ISSN (Online): | 2053-230X |
Published Online: | 21 April 2012 |
University Staff: Request a correction | Enlighten Editors: Update this record