Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein

Carter, S.D. , Barr, J.N. and Edwards, T.A. (2012) Expression, purification and crystallization of the Crimean-Congo haemorrhagic fever virus nucleocapsid protein. Acta Crystallographica. Section F: Structural Biology Communications, 68(5), pp. 569-573. (doi: 10.1107/s1744309112009736) (PMID:22691790) (PMCID:PMC3374515)

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Abstract

Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, [beta] = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Carter, Dr Stephen
Authors: Carter, S.D., Barr, J.N., and Edwards, T.A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Acta Crystallographica. Section F: Structural Biology Communications
Publisher:International Union of Crystallography
ISSN:1744-3091
ISSN (Online):2053-230X
Published Online:21 April 2012

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