Guidelines for reporting methods to estimate metabolic rates by aquatic intermittent-flow respirometry

Killen, S. S. et al. (2021) Guidelines for reporting methods to estimate metabolic rates by aquatic intermittent-flow respirometry. Journal of Experimental Biology, 224(18), jeb242522. (doi: 10.1242/jeb.242522) (PMID:34520540) (PMCID:PMC8467026)

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Abstract

Interest in the measurement of metabolic rates is growing rapidly, because of the importance of metabolism in advancing our understanding of organismal physiology, behaviour, evolution and responses to environmental change. The study of metabolism in aquatic animals is undergoing an especially pronounced expansion, with more researchers utilising intermittent-flow respirometry as a research tool than ever before. Aquatic respirometry measures the rate of oxygen uptake as a proxy for metabolic rate, and the intermittent-flow technique has numerous strengths for use with aquatic animals, allowing metabolic rate to be repeatedly estimated on individual animals over several hours or days and during exposure to various conditions or stimuli. There are, however, no published guidelines for the reporting of methodological details when using this method. Here, we provide the first guidelines for reporting intermittent-flow respirometry methods, in the form of a checklist of criteria that we consider to be the minimum required for the interpretation, evaluation and replication of experiments using intermittent-flow respirometry. Furthermore, using a survey of the existing literature, we show that there has been incomplete and inconsistent reporting of methods for intermittent-flow respirometry over the past few decades. Use of the provided checklist of required criteria by researchers when publishing their work should increase consistency of the reporting of methods for studies that use intermittent-flow respirometry. With the steep increase in studies using intermittent-flow respirometry, now is the ideal time to standardise reporting of methods, so that – in the future – data can be properly assessed by other scientists and conservationists.

Item Type:Articles
Additional Information:S.S.K. was supported by a Natural Environment Research Council Standard Grant NE/T008334/1. E.A.F.C. was supported by the Carlsberg Foundation (Carlsbergfondet CF19-0400). T.N. was supported by funding from the European Union’s Horizon 2020 Framework Programme under the Marie Skłodowska-Curie grant agreement no. 713683. L.Z. was supported by the Austrian Science Fund, FWF, Lise Meitner Program (Project M2742 BBL). J.J.H.N. was supported by funding from the European Union’s Horizon 2020 Framework Programme under the Marie Skłodowska-Curie grant agreement no. 839039. Open access funding provided by University of Glasgow.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cortese, Ms Daphne and Zavorka, Dr Libor and Nati, Miss Julie and Cotgrove, Lucy and Munson, Ms Amelia and Christensen, Dr Emil and Killen, Professor Shaun and Crespel, Dr Amelie and Papatheodoulou, Magdalene and Norin, Dr Tommy
Creator Roles:
Killen, S.Conceptualization, Methodology, Data curation, Formal analysis, Writing – original draft, Writing – review and editing, Visualization
Christensen, E.Methodology, Data curation, Writing – review and editing
Cortese, D.Methodology, Data curation, Writing – review and editing, Visualization
Zavorka, L.Methodology, Data curation, Formal analysis, Writing – review and editing
Norin, T.Methodology, Data curation, Writing – review and editing
Cotgrove, L.Methodology, Data curation, Writing – review and editing
Crespel, A.Methodology, Data curation, Writing – review and editing
Munson, A.Methodology, Data curation, Writing – review and editing
Nati, J.Methodology, Data curation, Writing – review and editing
Papatheodoulou, M.Methodology, Data curation, Writing – review and editing
Authors: Killen, S. S., Christensen, E. A.F., Cortese, D., Zavorka, L., Norin, T., Cotgrove, L., Crespel, A., Munson, A., Nati, J. J.H., Papatheodoulou, M., and McKenzie, D. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Experimental Biology
Publisher:The Company of Biologists
ISSN:0022-0949
ISSN (Online):1477-9145
Published Online:14 September 2021
Copyright Holders:Copyright © 2021 The Company of Biologists
First Published:First published in Journal of Experimental Biology 224(18):jeb242522
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
308473Effects of Climate-Change Associated Stressors on Fish Social BehavioursShaun KillenNatural Environment Research Council (NERC)NE/T008334/1Institute of Biodiversity, Animal Health and Comparative Medicine