Anillin/Mid1p interacts with the ESCRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast

Rezig, I. M., Yaduma, W. G., Gould, G. W. and McInerny, C. J. (2021) Anillin/Mid1p interacts with the ESCRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast. Cell Cycle, 20(18), pp. 1845-1860. (doi: 10.1080/15384101.2021.1962637) (PMID:34382912) (PMCID:PMC8525990)

Abstract

Cytokinesis is the final stage of the cell cycle which separates cellular constituents to produce two daughter cells. Using the fission yeast Schizosaccharomyces pombe we have investigated the role of various classes of proteins involved in this process. Central to these is anillin/Mid1p which forms a ring-like structure at the cell equator that predicts the site of cell separation through septation in fission yeast. Here we demonstrate a direct physical interaction between Mid1p and the endosomal sorting complex required for transport (ESCRT)-associated protein Vps4p, a genetic interaction of the mid1 and vps4 genes essential for cell viability, and a requirement of Vps4p for the correct cellular localization of Mid1p. Furthermore, we show that Mid1p is phosphorylated by aurora kinase, a genetic interaction of the mid1 and the aurora kinase ark1 genes is essential for cell viability, and that Ark1p is also required for the correct cellular localization of Mid1p. We mapped the sites of phosphorylation of Mid1p by human aurora A and the polo kinase Plk1 and assessed their importance in fission yeast by mutational analysis. Such analysis revealed serine residues S332, S523 and S531 to be required for Mid1p function and its interaction with Vps4p, Ark1p and Plo1p. Combined these data suggest a physical interaction between Mid1p and Vps4p important for cytokinesis, and identify phosphorylation of Mid1p by aurora and polo kinases as being significant for this process.

• Item Type: Articles
• Additional Information: This work was supported by the Tertiary Education Trust Fund (TETFUND) and Adamawa State College of Education, Nigeria studentship [grant number TETF/DASTD/COE/HONG/ASTD/2016/Vol.1] to WGY.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gould, Professor Gwyn and Yaduma, Mr Wandiahyel and Rezig, Miss Imane and McInerny, Dr Chris
• Authors: Rezig, I. M., Yaduma, W. G., Gould, G. W., and McInerny, C. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Medical Veterinary and Life Sciences > School of Life Sciences
• Journal Name: Cell Cycle
• Publisher: Taylor and Francis
• ISSN: 1538-4101
• ISSN (Online): 1551-4005
• Published Online: 12 August 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Cell Cycle 20(18): 1845-1860
• Publisher Policy: Reproduced under a Creative Commons License