Ylinen, L. M. J., Schaller, T., Price, A., Fletcher, A. J., Noursadeghi, M., James, L. C. and Towers, G. J. (2010) Cyclophilin A levels dictate infection efficiency of human immunodeficiency virus type 1 capsid escape mutants A92E and G94D. Journal of Virology, 83(4), pp. 2044-2047. (doi: 10.1128/jvi.01876-08) (PMID:19073742) (PMCID:PMC2643744)
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Abstract
Cyclophilin A (CypA) is an important human immunodeficiency virus type 1 (HIV-1) cofactor in human cells. HIV-1 A92E and G94D capsid escape mutants arise during CypA inhibition and in certain cell lines are dependent on CypA inhibition. Here we show that dependence on CypA inhibition is due to high CypA levels. Restricted HIV-1 is stable, and remarkably, restriction is augmented by arresting cell division. Nuclear entry is not inhibited. We propose that high CypA levels and capsid mutations combine to disturb uncoating, leading to poor infectivity, particularly in arrested cells. Our data suggest a role for CypA in uncoating the core of HIV-1 to facilitate integration.
Item Type: | Articles |
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Additional Information: | This work was supported by Wellcome Trust fellowships to G.J.T. (WT076608) and M.N. (WT077161) and the Medical Research Council. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Fletcher, Dr Adam |
Authors: | Ylinen, L. M. J., Schaller, T., Price, A., Fletcher, A. J., Noursadeghi, M., James, L. C., and Towers, G. J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Journal of Virology |
Publisher: | American Society for Microbiology |
ISSN: | 0022-538X |
ISSN (Online): | 1098-5514 |
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