Mansouri, D. , McSorley, S. T. , Park, J. H. , Orange, C., Horgan, P. G. , McMillan, D. C. and Edwards, J. (2021) The inflammatory microenvironment in screen-detected premaligant adenomatous polyps: early results from the integrated technologies for improved polyp surveillance (INCISE) project. European Journal of Gastroenterology and Hepatology, 33(7), pp. 983-989. (doi: 10.1097/MEG.0000000000002202) (PMID:34034277)
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Abstract
Introduction: Around 40% of patients who attend colonoscopy following a positive stool screening test have adenomatous polyps. Identifying which patients have a higher propensity for malignant transformation is currently poorly understood. The aim of the present study was to assess whether the type and intensity of inflammatory infiltrate differ between screen-detected adenomas with high-grade dysplasia (HGD) and low-grade dysplasia (LGD). Methods: A representative sample of 207 polyps from 134 individuals were included from a database of all patients with adenomas detected through the first round of the Scottish Bowel Screening Programme in NHS Greater Glasgow and Clyde (April 2009-April 2011). Inflammatory cell phenotype infiltrate was assessed by immunohistochemistry for CD3+, CD8+, CD45+ and CD68+ in a semi-quantitative manner at 20x resolution. Immune-cell infiltrate was graded as absent, weak, moderate or strong. Patient and polyp characteristics and inflammatory infiltrate were then compared between HGD and LGD polyps. Results: CD3+ infiltrate was significantly higher in HGD polyps compared to LGD polyps (74 vs. 69%; P < 0.05). CD8+ infiltrate was significantly higher in HGD polyps compared to LGD polyps (36 vs. 13%; P < 0.001) whereas CD45+ infiltrate was not significantly different (69 vs. 64%; P = 0.401). There was no significant difference in CD68+ infiltrate (P = 0.540) or total inflammatory cell infiltrate (calculated from CD3+ and CD68+) (P = 0.226). Conclusions: This study reports an increase in CD3+ and CD8+ infiltrate in HGD colonic adenomas when compared to LGD adenomas. It may therefore have a use in the prognostic stratification and treatment of dysplastic polyps.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Park, Mr James and Mansouri, Mr David and Horgan, Professor Paul and McSorley, Dr Stephen and McMillan, Professor Donald and Orange, Miss Clare and Edwards, Professor Joanne |
| Authors: | Mansouri, D., McSorley, S. T., Park, J. H., Orange, C., Horgan, P. G., McMillan, D. C., and Edwards, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | European Journal of Gastroenterology and Hepatology |
| Publisher: | Lippincott, Williams & Wilkins |
| ISSN: | 0954-691X |
| ISSN (Online): | 1473-5687 |
| Published Online: | 25 May 2021 |
| Copyright Holders: | Copyright © 2021 Wolters Kluwer Health, Inc |
| First Published: | First published in European Journal of Gastroenterology and Hepatology 33(7): 983-989 |
| Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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