A rapid variant-tolerant reverse transcription loop-mediated isothermal amplification assay for point of care detection of HIV-1

Li, Y. et al. (2021) A rapid variant-tolerant reverse transcription loop-mediated isothermal amplification assay for point of care detection of HIV-1. Analyst, 146(17), pp. 5347-5356. (doi: 10.1039/D1AN00598G) (PMID:34323889)

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Abstract

Human immunodeficiency virus (HIV) continues to cause a major burden on public health globally with on-going increases in numbers of new infections each year. Rapid and sensitive point-of-care test (POCT) allows timely interventions, essential to control the spread of the AIDS disease, although the highly variable nature of the virus, resulting in the evolution of many subtypes and inter-subtype recombinants, poses important challenges in its diagnosis. Here we describe a variant-tolerant reverse-transcription RT-LAMP amplification of the virus’s INT gene, providing a simple to use, rapid (<30 min) in vitro diagnostic test with a limit of detection <18 copies/reaction. The assay was first validated in clinical studies of patient samples, using both established RT-LAMP and RT-qPCR assays for reference, with results showing that this new variant-tolerant HIV-1 RT-LAMP diagnostic test is highly sensitive without compromising upon its high specificity for the HIV-1 subtypes. The diagnostic test was subsequently configured within an easy-to-read paper microfluidic POC lateral flow test and was validated clinically using patient samples, demonstrating its future potential for use in timely, effective, low costing HIV diagnostic tests in global regions where healthcare resources may be limited.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Dr Julien and Xu, Mr Gaolian
Authors: Li, Y., Chen, X., Zhao, Y., Wan, Z., Zeng, Y., Ma, Y., Zhou, L., Xu, G., Reboud, J., Cooper, J. M., and Zhang, C.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Analyst
Publisher:Royal Society of Chemistry
ISSN:0003-2654
ISSN (Online):1364-5528
Published Online:02 July 2021
Copyright Holders:Copyright © 2021 The Royal Society of Chemistry
First Published:First published in Analyst 146(17): 5347-5356
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
300573Novel low cost diagnostic tools and their impact in AfricaJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/R01437X/1ENG - Biomedical Engineering