Screening of biomineralization using microfluidics

Yin, H. , Bozhi, J., Dobson, P.S. , Mosbahi, K., Glidle, A., Gadegaard, N. , Freer, A., Cooper, J.M. and Cusack, M. (2009) Screening of biomineralization using microfluidics. Analytical Chemistry, 81(1), pp. 473-478. (doi:10.1021/ac801980b)

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Publisher's URL: http://dx.doi.org/10.1021/ac801980b

Abstract

Biomineralization is the process where biological systems produce well-defined composite structures such as shell, teeth, and bones. Currently, there is substantial momentum to investigate the processes implicated in biomineralization and to unravel the complex roles of proteins in the control of polymorph switching. An understanding of these processes may have wide-ranging significance in health care applications and in the development of advanced materials. We have demonstrated a microfluidic approach toward these challenges. A reversibly sealed T-junction microfluidic device was fabricated to investigate the influence of extrapallial (EP) fluid proteins in polymorph control of crystal formation in mollusk shells. A range of conditions were investigated on chip, allowing fast screening of various combinations of ion, pH, and protein concentrations. The dynamic formation of crystals was monitored on chip and combined with in situ Raman to reveal the polymorph in real time. To this end, we have demonstrated the unique advantages of this integrated approach in understanding the processes involved in biomineralization and revealing information that is impossible to obtain using traditional methods.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Yin, Professor Huabing and Cusack, Professor Maggie and Gadegaard, Professor Nikolaj and Glidle, Dr Andrew and Mosbahi, Dr Khedidja and Dobson, Dr Phil
Authors: Yin, H., Bozhi, J., Dobson, P.S., Mosbahi, K., Glidle, A., Gadegaard, N., Freer, A., Cooper, J.M., and Cusack, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
College of Science and Engineering > School of Geographical and Earth Sciences
Journal Name:Analytical Chemistry
ISSN:0003-2700
ISSN (Online):1520-6882
Published Online:20 November 2008

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
452471Understanding polymorph production and control in calcite/aragonite biomineralsAndrew FreerBiotechnology and Biological Sciences Research Council (BBSRC)BB/E025110/1Chemistry