Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice

da Silva, R. d. N. O., Santos-Eichler, R. A., Dias, C., Rodrigues, S. F., Skiba, D. S., Landgraf, R. G., de Carvalho, M. H. C., Guzik, T., Fock, R. A. and Akamine, E. H. (2021) Immune spleen cells attenuate the inflammatory profile of the mesenteric perivascular adipose tissue in obese mice. Scientific Reports, 11, 11153. (doi: 10.1038/s41598-021-90600-0) (PMID:34045574) (PMCID:PMC8160359)

Abstract

The perivascular adipose tissue (PVAT) differs from other fat depots and exerts a paracrine action on the vasculature. The spleen has an important role in the immune response, and it was observed to have either a protective role or a contribution to obesity-related diseases. However, the relation between spleen and PVAT is elusive in obesity. We investigated the role of spleen in the inflammatory profile of the mesenteric PVAT (mPVAT) from mice fed a high-fat diet (HFD) for 16 weeks. Male C57Bl/6 mice were sham-operated or splenectomized (SPX) and fed a HFD for 16 weeks. mPVAT morphology was evaluated by hematoxylin and eosin staining, infiltrated immune cells were evaluated by flow cytometry, inflammatory cytokines were evaluated by ELISA and the splenic cell chemotaxis mediated by mPVAT was evaluated using a transwell assay. In SPX mice, HFD induced adipocyte hypertrophy and increased immune cell infiltration and proinflammatory cytokine levels in mPVAT. However, none of these effects were observed in mPVAT from sham-operated mice. Spleen from HFD fed mice presented reduced total leukocytes and increased inflammatory markers when compared to the spleen from control mice. Chemotaxis of spleen cells mediated by mPVAT of HFD fed mice was reduced in relation to standard diet fed mice. The spleen protects mPVAT against the effects of 16-week HFD. This information was missing, and it is important because PVAT is different from other fat depots and data cannot be extrapolated from any type of adipose tissue to PVAT.

• Item Type: Articles
• Additional Information: Funding: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [Grant Number 11/51254-7], Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) [Grant Number 471918/2010-5], and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [Finance Code 001] have funded this study. RNOS was the recipient of a fellowship from CNPq, and CD and SFR from FAPESP. RNOS received a fellowship from Programa de Doutorado Sanduíche no Exterior (PDSE) of CAPES [Grant Number 88881.132199/2016-01].
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Guzik, Professor Tomasz and Skiba, Mr Dominik
• Authors: da Silva, R. d. N. O., Santos-Eichler, R. A., Dias, C., Rodrigues, S. F., Skiba, D. S., Landgraf, R. G., de Carvalho, M. H. C., Guzik, T., Fock, R. A., and Akamine, E. H.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Scientific Reports
• Publisher: Nature Research
• ISSN: 2045-2322
• ISSN (Online): 2045-2322
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Scientific Reports 11: 11153
• Publisher Policy: Reproduced under a Creative Commons License