Guard cell endomembrane Ca2+-ATPases underpin a ‘carbon memory’ of photosynthetic assimilation that impacts on water-use efficiency

Jezek, M. et al. (2021) Guard cell endomembrane Ca2+-ATPases underpin a ‘carbon memory’ of photosynthetic assimilation that impacts on water-use efficiency. Nature Plants, 7(9), pp. 1301-1313. (doi: 10.1038/s41477-021-00966-2) (PMID:34326530)

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Abstract

Stomata of most plants close to preserve water when the demand for CO2 by photosynthesis is reduced. Stomatal responses are slow compared with photosynthesis, and this kinetic difference erodes assimilation and water-use efficiency under fluctuating light. Despite a deep knowledge of guard cells that regulate the stoma, efforts to enhance stomatal kinetics are limited by our understanding of its control by foliar CO2. Guided by mechanistic modelling that incorporates foliar CO2 diffusion and mesophyll photosynthesis, here we uncover a central role for endomembrane Ca2+ stores in guard cell responsiveness to fluctuating light and CO2. Modelling predicted and experiments demonstrated a delay in Ca2+ cycling that was enhanced by endomembrane Ca2+-ATPase mutants, altering stomatal conductance and reducing assimilation and water-use efficiency. Our findings illustrate the power of modelling to bridge the gap from the guard cell to whole-plant photosynthesis, and they demonstrate an unforeseen latency, or ‘carbon memory’, of guard cells that affects stomatal dynamics, photosynthesis and water-use efficiency.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hills, Mr Adrian and Blatt, Professor Michael and Alvim, Fernanda Aparecida and Jezek, Mareike and Donald, Miss Naomi and Wang, Dr Yizhou
Authors: Jezek, M., Silva-Alvim, F. A.L., Hills, A., Donald, N., Ishka, M. R., Shadbolt, J., He, B., Lawson, T., Harper, J. F., Wang, Y., Lew, V. L., and Blatt, M. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Nature Plants
Publisher:Nature Research
ISSN:2055-026X
ISSN (Online):2055-0278
Published Online:29 July 2021
Copyright Holders:Copyright © 2021 Springer Nature Limited
First Published:First published in Nature Plants 7(9): 1301-1313
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190663Stomatal-based systems analysis of water use efficiencyMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L001276/1MCSB - Plant Sciences
170646Developing a synthetic approach to manipulating guard cell membrane transport and stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/L019205/1MCSB - Plant Sciences
170599Analysing GORK clustering for enhanced stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/M001601/1MCSB - Plant Sciences
172696Synthesis of Microcompartments in Plants for Enhanced Carbon FixationMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/N01832X/1MCSB - Plant Sciences