Junctional adhesion molecule-A on dendritic cells regulates Th1 differentiation

Bonilha, C. S., Benson, R. A. , Scales, H. S. , Brewer, J. M. and Garside, P. (2021) Junctional adhesion molecule-A on dendritic cells regulates Th1 differentiation. Immunology Letters, 235, pp. 32-40. (doi: 10.1016/j.imlet.2021.05.001) (PMID:34000305)

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Abstract

The junctional adhesion molecule-A (JAM-A) is an adhesion molecule present in the surface of several cell types, such as endothelial cells and leukocytes as well as Dendritic Cells (DC). Given the potential relevance of JAM-A in diverse pathological conditions such as inflammatory diseases and cancer, we investigated the role of JAM-A in CD4+ T cell priming. We demonstrate that JAM-A is present in the immunological synapse formed between T cells and DC during priming. Furthermore, an antagonistic anti-JAM-A mAb could disrupt the interaction between CD4+ T cell and DC. Antagonism of JAM-A also attenuated T cell activation and proliferation with a decrease in T-bet expression and increased IL-6 and IL-17 secretion. These findings demonstrate a functional role for JAM-A in interactions between CD4+ T cells and DCs during T cell priming as a positive regulator of Th1 differentiation.

Item Type:Articles
Additional Information:CSB was supported by CAPES Foundation, an agency under the Ministry of Education of Brazil (grant number 88881.129556/2016-01). CSB, RAB, HES, JMB and PG were supported by the Arthritis Research UK (ARUK) program grant number 19788 and the Innovative Medicines Initiative EU-funded project Be The Cure (BTCURE) [grant number 115142-2]. JMB and PG were also supported by the Research into Inflammatory Arthritis Center Versus Arthritis (RACE) (grant number 22072) .
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Benson, Dr Robert and Garside, Professor Paul and Brewer, Professor James and Scales, Dr Hannah and Bonilha, Mr Caio
Creator Roles:
Bonilha, C. S.Conceptualization, Investigation, Formal analysis, Writing – original draft
Benson, R. A.Conceptualization, Investigation, Writing – review and editing
Scales, H.Project administration, Writing – review and editing
Brewer, J. M.Supervision, Funding acquisition, Writing – review and editing
Garside, P.Funding acquisition, Writing – review and editing
Authors: Bonilha, C. S., Benson, R. A., Scales, H. S., Brewer, J. M., and Garside, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Immunology Letters
Publisher:Elsevier
ISSN:0165-2478
ISSN (Online):1879-0542
Published Online:14 May 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Immunology Letters 235:32-40
Publisher Policy:Reproduced under a Creative Commons Licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
166480Defining the Role of Cell Migration in the Initiation and Regulation of Pathogenesis in Inflammatory ArthritisPaul GarsideVersus Arthritis (ARTRESUK)MP/19788III - Immunology
303229RACE RenewalIain McInnesVersus Arthritis (ARTRESUK)NW/22072III - Immunology