Urinary 11-dehydrothromboxane B₂ concentrations in 20 dogs with primary immune-mediated hemolytic anemia

Conway, E. A. , Evans, N. P. and Ridyard, A. E. (2022) Urinary 11-dehydrothromboxane B₂ concentrations in 20 dogs with primary immune-mediated hemolytic anemia. Journal of Veterinary Internal Medicine, 36(1), pp. 86-96. (doi: 10.1111/jvim.16322) (PMID:34859495) (PMCID:PMC8783321)

Abstract

Background: Thromboembolic disease is a major cause of mortality in dogs with immune-mediated hemolytic anemia (IMHA). At present, no reliable biomarkers of individual patient thrombotic risk are available. In human medicine, increased urinary thromboxane concentrations have utility as markers of prothrombotic tendency in various situations. Hypothesis/Objectives: First, to determine if urinary 11-dehydrothromboxane B2 (u11-dTXB) concentrations are increased in dogs with primary IMHA compared to normal dogs; second, to assess whether u11-dTXB concentration is associated with survival, known prognostic indicators, or frequency of thrombosis in dogs with IMHA. Animals: Twenty client-owned dogs diagnosed with primary IMHA and 17 healthy dogs volunteered by hospital staff. Methods: Prospective case-control study. A previously validated ELISA was used to measure urine 11-dTXB concentrations, which were normalized to urine creatinine concentration (u11-dTXB:Cr). Samples were obtained at presentation from patients with primary IMHA. Standard clincopathological data at baseline and survival data were collected. Urinary 11-dTXB:Cr was compared between outcome subgroups, and correlated with known markers of disease severity. Results: Baseline u11-dTXB:Cr was significantly higher in dogs with IMHA than in healthy dogs (median, 3.75; range, 0.83-25.36 vs 0.65; 0.24-2.57; P = .003) but did not differ between dogs with IMHA that survived and did not survive to 30 days after presentation, nor between dogs with and without clinical suspicion of thrombotic disease. Conclusions and Clinical Importance: Urinary 11-dTXB:Cr is increased in dogs with IMHA compared to healthy controls, consistent with a prothrombotic state. However, in this IMHA population u11-dTXB:Cr was not associated with survival or suspected thrombosis.

• Item Type: Articles
• Additional Information: Funding for this study was provided by University of Glasgow Vet Fund, number Vet Fund 2016.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Conway, Dr Elizabeth and Ridyard, Ms Alison and Evans, Professor Neil
• Authors: Conway, E. A., Evans, N. P., and Ridyard, A. E.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Veterinary Internal Medicine
• Publisher: Wiley
• ISSN: 0891-6640
• ISSN (Online): 1939-1676
• Published Online: 03 December 2021
• Copyright Holders: Copyright © 2021 The Authors
• First Published: First published in Journal of Veterinary Internal Medicine 36(1): 86-96
• Publisher Policy: Reproduced under a Creative Commons License