The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides

Houssen, W. E., Koehnke, J. , Zollman, D., Vendome, J., Raab, A., Smith, M. C. M., Naismith, J. H. and Jaspars, M. (2012) The discovery of new cyanobactins from Cyanothece PCC 7425 defines a new signature for processing of patellamides. ChemBioChem, 13(18), pp. 2683-2689. (doi: 10.1002/cbic.201200661) (PMID:23169461)

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Abstract

Cyanobactins, including patellamides, are a group of cyanobacterial post‐translationally modified ribosomal cyclic peptides. The final product should in theory be predictable from the sequence of the precursor peptide and the associated tailoring enzymes. Understanding the mechanism and recognition requirements of these enzymes will allow their rational engineering. We have identified three new cyanobactins from a Cyanothece PCC 7425 culture subjected to a heat shock. One of these compounds revealed a novel signature signal for ThcA, the subtilisin‐like serine protease that is homologous to the patellamide protease PatA. The crystal structure of the latter and modelling studies allow rationalisation of the recognition determinants for both enzymes, consistent with the ribosomal biosynthetic origin of the new compounds.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Koehnke, Dr Jesko
Authors: Houssen, W. E., Koehnke, J., Zollman, D., Vendome, J., Raab, A., Smith, M. C. M., Naismith, J. H., and Jaspars, M.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:ChemBioChem
Publisher:Wiley-VCH Verlag
ISSN:1439-4227
ISSN (Online):1439-7633

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