New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography

Schorb, M., Gaechter, L., Avinoam, O., Sieckmann, F., Clarke, M. , Bebeacua, C., Bykov, Y. S., Sonnen, A. F.-P., Lihl, R. and Briggs, J. A.G. (2017) New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography. Journal of Structural Biology, 197(2), pp. 83-93. (doi: 10.1016/j.jsb.2016.06.020) (PMID:27368127) (PMCID:PMC5287355)

Abstract

Correlative light and electron microscopy allows features of interest defined by fluorescence signals to be located in an electron micrograph of the same sample. Rare dynamic events or specific objects can be identified, targeted and imaged by electron microscopy or tomography. To combine it with structural studies using cryo-electron microscopy or tomography, fluorescence microscopy must be performed while maintaining the specimen vitrified at liquid-nitrogen temperatures and in a dry environment during imaging and transfer. Here we present instrumentation, software and an experimental workflow that improves the ease of use, throughput and performance of correlated cryo-fluorescence and cryo-electron microscopy. The new cryo-stage incorporates a specially modified high-numerical aperture objective lens and provides a stable and clean imaging environment. It is combined with a transfer shuttle for contamination-free loading of the specimen. Optimized microscope control software allows automated acquisition of the entire specimen area by cryo-fluorescence microscopy. The software also facilitates direct transfer of the fluorescence image and associated coordinates to the cryo-electron microscope for subsequent fluorescence-guided automated imaging. Here we describe these technological developments and present a detailed workflow, which we applied for automated cryo-electron microscopy and tomography of various specimens.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Clarke, Dr Mairi
• Authors: Schorb, M., Gaechter, L., Avinoam, O., Sieckmann, F., Clarke, M., Bebeacua, C., Bykov, Y. S., Sonnen, A. F.-P., Lihl, R., and Briggs, J. A.G.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Journal of Structural Biology
• Publisher: Elsevier
• ISSN: 1047-8477
• ISSN (Online): 1095-8657
• Published Online: 28 June 2016
• Copyright Holders: Copyright © 2016 The Authors
• First Published: First published in Journal of Structural Biology 197(2): 83-93
• Publisher Policy: Reproduced under a Creative Commons License