Wan, W., Kolesnikova, L., Clarke, M. , Koehler, A., Noda, T., Becker, S. and Briggs, J. A.G. (2017) Structure and assembly of the Ebola virus nucleocapsid. Nature, 551(7680), pp. 394-397. (doi: 10.1038/nature24490) (PMID:29144446) (PMCID:PMC5714281)
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Abstract
Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever1. Filoviruses are within the order Mononegavirales2, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein–nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein3,4,5,6, and cryo-electron microscopy of nucleocapsid7,8,9,10,11,12 or nucleocapsid-like structures11,12,13. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended α-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Clarke, Dr Mairi |
| Authors: | Wan, W., Kolesnikova, L., Clarke, M., Koehler, A., Noda, T., Becker, S., and Briggs, J. A.G. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Nature |
| Publisher: | Nature Research |
| ISSN: | 0028-0836 |
| ISSN (Online): | 1476-4687 |
| Published Online: | 08 November 2017 |
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