Existence of multiple ESBL genes among phenotypically confirmed ESBL producing Klebsiella pneumoniae and Escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania

Silago, V., Kovacs, D., Samson, H., Seni, J., Matthews, L. , Oravcová, K. , Lupindu, A. M., Hoza, A. S. and Mshana, S. E. (2021) Existence of multiple ESBL genes among phenotypically confirmed ESBL producing Klebsiella pneumoniae and Escherichia coli concurrently isolated from clinical, colonization and contamination samples from neonatal units at Bugando Medical Center, Mwanza, Tanzania. Antibiotics, 10(5), 476. (doi: 10.3390/antibiotics10050476) (PMID:33919117) (PMCID:PMC8143173)

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Abstract

The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing K. pneumoniae (ESBL-KP) and E. coli (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (blaCTX-M, blaTEM, and blaSHV) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at −80 °C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (blaCTX-M, blaTEM, and blaSHV) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.

Item Type:Articles
Keywords:Antimicrobial resistance, extended-spectrum beta-lactamase, Escherichia coli, Klebsiella pneumoniae.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Oravcova, Dr Katarina and Matthews, Professor Louise and Kovacs, Dorottya
Creator Roles:
Kovacs, D.Investigation, Methodology, Writing – review and editing
Matthews, L.Formal analysis, Methodology, Writing – review and editing
Oravcová, K.Formal analysis, Methodology, Writing – review and editing
Authors: Silago, V., Kovacs, D., Samson, H., Seni, J., Matthews, L., Oravcová, K., Lupindu, A. M., Hoza, A. S., and Mshana, S. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Antibiotics
Publisher:MDPI
ISSN:2079-6382
ISSN (Online):2079-6382
Published Online:21 April 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Antibiotics 10(5): 476
Publisher Policy:Reproduced under a Creative Commons License

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