Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan

Chabane, K., Abdalla, O., Sayed, H. and Valkoun, J. (2007) Assessment of EST-microsatellites markers for discrimination and genetic diversity in bread and durum wheat landraces from Afghanistan. Genetic Resources and Crop Evolution, 54(5), pp. 1073-1080. (doi: 10.1007/s10722-006-9193-2)

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Abstract

Accurate and reliable means for identification are necessary to assess the discrimination between landraces of tetraploid wheat [T.␣turgidum L. subsp. durum (Desf.) Husn.] and hexaploid wheat (T. aestivum L. em. Thell.). In Afghanistan, farmers usually cultivate mixed landraces, and thus distinction between bread and durum is difficult. A set of 18 microsatellites derived from the DuPont EST-database were used to describe genetic diversity in a sample of 82 Afghan wheat landraces. A total of 101 alleles were detected, with allele number per locus ranging from 2 to 13, and a mean allele number of 6.31. The percentage of polymorphic loci was 89%. The EST-SSRs markers showed different level of gene diversity: the highest Polymorphism Information Content value (0.921) was observed with DuPw 221. Our results demonstrated that with a reasonable number of expressed sequences target microsatellites (EST-SSRs) it is possible to discriminate between T. durum and T. aestivum species of wheat germplasm. Our results showed that EST-databases could be a useful source for species-specific markers and have the potential for new genic microsatellites markers that could enhance screening germplasm in gene banks.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sayed, Dr Haitham
Authors: Chabane, K., Abdalla, O., Sayed, H., and Valkoun, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Genetic Resources and Crop Evolution
Publisher:Springer
ISSN:0925-9864
ISSN (Online):1573-5109
Published Online:24 January 2007

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