Eaglesfield, R., Madsen, M. A., Sanyal, S., Reboud, J. and Amtmann, A. (2021) Co-translational recruitment of ribosomes in minimal protocells recreates a translocon-independent mechanism of proteorhodopsin biogenesis. iScience, 24(5), 102429. (doi: 10.1016/j.isci.2021.102429) (PMID:33997704) (PMCID:PMC8102411)
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Abstract
The emergence of lipid membranes and embedded proteins was essential for the evolution of cells. Translocon complexes mediate co-translational recruitment and membrane insertion of nascent proteins, but they already contain membrane-integral proteins. Therefore, a simpler mechanism must exist, enabling spontaneous membrane integration while preventing aggregation of un-chaperoned protein in aqueous phase. Here, we used giant unilamellar vesicles encapsulating minimal translation components to systematically interrogate the requirements for insertion of the model protein proteorhodopsin (PR) – a structurally ubiquitous membrane protein. We show that the N-terminal hydrophobic domain of PR is both necessary and sufficient for co-translational recruitment of ribosomes to the membrane and subsequent membrane insertion of PR. Insertion of N-terminally truncated PR was restored by artificially attaching ribosomes to the membrane. Our findings offer a self-sufficient protein-inherent mechanism as a possible explanation for effective membrane protein biogenesis in a ‘pre-translocon’ era, and they offer new opportunities for generating artificial cells.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was funded by the University of Glasgow (Lord Kelvin Adam Smith (LKAS) PhD scholarship to R.E.),by the Biotechnology and Biological Sciences Research Council (BBSRC; BB/R019894/1 to M.A.M and A.A.), and by the Swedish Research Council grants (2016-06264, 2018-05946 and 2018-05498 to S.S.). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Madsen, Dr Mary Ann and Amtmann, Professor Anna and Reboud, Professor Julien and Eaglesfield, Mr Ross |
| Authors: | Eaglesfield, R., Madsen, M. A., Sanyal, S., Reboud, J., and Amtmann, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | iScience |
| Publisher: | Elsevier (Cell Press) |
| ISSN: | 2589-0042 |
| ISSN (Online): | 2589-0042 |
| Published Online: | 14 April 2021 |
| Copyright Holders: | Copyright © 2021 The Authors |
| First Published: | First published in iScience 24(5): 102429 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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