Loss of APC induces polyploidy as a result of a combination of defects in mitosis and apoptosis

Dikovskaya, D., Schiffmann, D., Newton, I., Oakley, A., Kroboth, K., Sansom, O. , Jamieson, T., Meniel, V., Clarke, A. and Nathke, I. (2007) Loss of APC induces polyploidy as a result of a combination of defects in mitosis and apoptosis. Journal of Cell Biology, 176(2), pp. 183-195. (doi:10.1083/jcb.200610099)

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Abstract

Mutations in the adenomatous polyposis coli (APC) tumor suppressor gene initiate a majority of colorectal cancers. Acquisition of chromosomal instability is an early event in these tumors. We provide evidence that the loss of APC leads to a partial loss of inter-kinetochore tension at metaphase and alters mitotic progression. Furthermore, we show that inhibition of APC in U2OS cells compromises the mitotic spindle checkpoint. This is accompanied by a decrease in the association of the checkpoint proteins Bub1 and BubR1 with kinetochores. Additionally, APC depletion reduced apoptosis. As expected from this combination of defects, tetraploidy and polyploidy are consequences of APC inhibition in vitro and in vivo. The removal of APC produced the same defects in HCT1 16 cells that have constitutively active P-catenin. These data show that the loss of APC immediately induces chromosomal instability as a result of a combination of mitotic and apoptotic defects. We suggest that these defects amplify each other to increase the incidence of tetra- and polyploidy in early stages of tumorigenesis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sansom, Professor Owen
Authors: Dikovskaya, D., Schiffmann, D., Newton, I., Oakley, A., Kroboth, K., Sansom, O., Jamieson, T., Meniel, V., Clarke, A., and Nathke, I.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Journal of Cell Biology
ISSN:1540-8140

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