Extracellular ATP causes ROCK I-dependent bleb formation in P2X(7)-transfected HEK293 cells

Morelli, A., Chiozzi, P., Chiesa, A., Ferrari, D., Sanz, J., Falzoni, S., Pinton, P., Rizzuto, R., Olson, M. and Di Virgilio, F. (2003) Extracellular ATP causes ROCK I-dependent bleb formation in P2X(7)-transfected HEK293 cells. Molecular Biology of the Cell, 14(7), pp. 2655-2664. (doi: 10.1091/mbc.02-04-0061)

Abstract

The P2X(7) ATP receptor mediates the cytotoxic effect of extracellular ATP. P2X(7)-dependent cell death is heralded by dramatic plasma membrane bleb formation. Membrane blebbing is a complex phenomenon involving as yet poorly characterized intracellular pathways. We have investigated the effect of extracellular ATP on HEK293 cells transfected with the cytotoxic/pore-forming P2X(7) receptor. Addition of ATP to P2X(7)-transfected, but not to wt P2X(7)-less, HEK293 cells caused massive membrane blebbing within 1-2 min. UTP, a nucleotide incapable of activating P2X(7), had no early effects on cell shape and bleb formation. Bleb formation triggered by ATP was reversible and required extracellular Ca2+ and an intact cytoskeleton. Furthermore, it was completely prevented by preincubation with the P2X(7) blocker oxidized ATP. It was recently observed that the ROCK protein is a key determinant of bleb formation. Preincubation of HEK293-P2X(7) cells with the ROCK blocker Y-27632 completely prevented P2X(7)-dependent blebbing. Although ATP triggered cleavage of the ROCK I isoform in P2X(7)- transfected HEK293 cells, the wide range caspase inhibitor z-VAD-fluoromethylketone had no effect. These observations suggest that P2X(7)-dependent plasma membrane blebbing depends on the activation of the serine/threonine kinase ROCK I.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Olson, Professor Michael
• Authors: Morelli, A., Chiozzi, P., Chiesa, A., Ferrari, D., Sanz, J., Falzoni, S., Pinton, P., Rizzuto, R., Olson, M., and Di Virgilio, F.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Molecular Biology of the Cell
• Publisher: American Society for Cell Biology
• ISSN: 1059-1524