Briggs, E. M., Warren, F. S.L., Matthews, K. R., McCulloch, R. and Otto, T. D. (2021) Application of single cell transcriptomics to kinetoplastid research. Parasitology, 148(10), pp. 1223-1236. (doi: 10.1017/S003118202100041X) (PMID:33678213) (PMCID:PMC8311972)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
237209.pdf - Published Version Available under License Creative Commons Attribution Non-commercial Share Alike. 1MB |
Abstract
Kinetoplastid parasites are responsible for both human and animal diseases across the globe where they have a great impact on health and economic well-being. Many species and life cycle stages are difficult to study due to limitations in isolation and culture, as well as to their existence as heterogeneous populations in hosts and vectors. Single-cell transcriptomics (scRNA-seq) has the capacity to overcome many of these difficulties, and can be leveraged to disentangle heterogeneous populations, highlight genes crucial for propagation through the life cycle, and enable detailed analysis of host–parasite interactions. Here, we provide a review of studies that have applied scRNA-seq to protozoan parasites so far. In addition, we provide an overview of sample preparation and technology choice considerations when planning scRNA-seq experiments, as well as challenges faced when analysing the large amounts of data generated. Finally, we highlight areas of kinetoplastid research that could benefit from scRNA-seq technologies.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Warren, Mr Felix and Briggs, Miss Emma and McCulloch, Professor Richard and Otto, Professor Thomas |
| Authors: | Briggs, E. M., Warren, F. S.L., Matthews, K. R., McCulloch, R., and Otto, T. D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
| Journal Name: | Parasitology |
| Publisher: | Cambridge University Press |
| ISSN: | 0031-1820 |
| ISSN (Online): | 1469-8161 |
| Published Online: | 08 March 2021 |
| Copyright Holders: | Copyright © 2021 The Authors |
| First Published: | First published in Parasitology 148(10):1223-1236 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric