Coleman, M., Sahai, E., Yeo, M., Bosch, M., Dewar, A. and Olson, M. (2001) Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I. Nature Cell Biology, 3(4), pp. 339-345. (doi: 10.1038/35070009)
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Abstract
The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Olson, Professor Michael |
| Authors: | Coleman, M., Sahai, E., Yeo, M., Bosch, M., Dewar, A., and Olson, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Nature Cell Biology |
| ISSN: | 1465-7392 |
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