RhoB and actin polymerization coordinate Src activation with endosome-mediated delivery to the membrane

Sandilands, E., Cans, C., Fincham, V., Brunton, V., Mellor, H., Prendergast, G., Norman, J. , Superti-Furga, G. and Frame, M. (2004) RhoB and actin polymerization coordinate Src activation with endosome-mediated delivery to the membrane. Developmental Cell, 7(6), pp. 855-869. (doi: 10.1016/j.devcel.2004.09.019)

Abstract

We have used a c-Src-GFP fusion protein to address the spatial control of Src activation and the nature of Src-associated intracellular structures during stimulus-induced transit to the membrane. Src is activated during transit, particularly in RhoB-containing cytoplasmic endosomes associated with the perinuclear recycling compartment. Knocking out RhoB or expressing a dominant-interfering Rab11 mutant suppresses both catalytic activation of Src and translocation of active kinase to peripheral membrane structures. In addition, the Src- and RhoB-containing endosomes harbor proteins involved in actin polymerization and filament assembly, for example Scar1, and newly polymerized actin can associate with these endosomes in a Src-dependent manner. This implies that Src may regulate an endosome-associated actin nucleation activity. In keeping with this, Src controls the actin dependence of RhoB endosome movement toward the plasma membrane. This work identifies RhoB as a component of "outside-in" signaling pathways that coordinate Src activation with translocation to transmembrane receptors.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Norman, Professor James
• Authors: Sandilands, E., Cans, C., Fincham, V., Brunton, V., Mellor, H., Prendergast, G., Norman, J., Superti-Furga, G., and Frame, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Developmental Cell
• ISSN: 1534-5807