Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks

Raza, A., Schulz, B. L., Nouwens, A., Jackson, L. A., Piper, E. K., James, P., Jonsson, N. N. and Tabor, A. E. (2021) Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks. Parasite Immunology, 43(7), e12836. (doi: 10.1111/pim.12836) (PMID:33843060)

Abstract

Previous studies have applied genomics and transcriptomics to identify immune and genetic markers as key indicator traits for cattle tick susceptibility/resistance; however, results differed between breeds, and there is lack of information on the use of host proteomics. Serum samples from Santa Gertrudis cattle (naïve and phenotyped over 105 days as tick‐resistant [TR] or tick‐susceptible [TS]) were used to conduct differential abundance analyses of protein profiles. Serum proteins were digested into peptides followed by identification and quantification using sequential window acquisition of all instances of theoretical fragment ion mass spectrometry. Before tick infestation, abundance of 28 proteins differed significantly (adjusted P < 10−5) between TR and TS. These differences were also observed following tick infestation (TR vs TS) with a further eight differentially abundant proteins in TR cattle, suggesting possible roles in adaptive responses. The intragroup comparisons (TS‐0 vs TS and TR‐0 vs TR) showed that tick infestation elicited quite similar responses in both groups of cattle, but with relatively stronger responses in TR cattle. Many of the significantly differentially abundant proteins in TR Santa Gertrudis cattle (before and after tick infestation) were associated with immune responses including complement factors, chemotaxis for immune cells and acute‐phase responses.

• Item Type: Articles
• Additional Information: The original Santa Gertrudis trial was funded by the Cooperative Research Centre (CRC) for Beef Genetic Technologies, Australia (Beef CRC, 2005‐2012). The current proteomics experiments were undertaken under a Meat and Livestock Australia Donor Company grant P.PSH.0798.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Jonsson, Professor Nicholas
• Authors: Raza, A., Schulz, B. L., Nouwens, A., Jackson, L. A., Piper, E. K., James, P., Jonsson, N. N., and Tabor, A. E.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Parasite Immunology
• Publisher: Wiley
• ISSN: 0141-9838
• ISSN (Online): 1365-3024
• Published Online: 11 April 2021
• Copyright Holders: Copyright © 2021 John Wiley & Sons, Ltd.
• First Published: First published in Parasite Immunology: e12836
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher