Identification of individual zebrafish (Danio rerio): a refined protocol for VIE tagging whilst considering animal welfare and the principles of the 3Rs

Rácz, A., Allan, B., Dwyer, T., Thambithurai, D., Crespel, A. and Killen, S. S. (2021) Identification of individual zebrafish (Danio rerio): a refined protocol for VIE tagging whilst considering animal welfare and the principles of the 3Rs. Animals, 11(3), 616. (doi: 10.3390/ani11030616) (PMID:33652779)

[img] Text
235883.pdf - Published Version
Available under License Creative Commons Attribution.

1MB

Abstract

In aquatic ecology, studies have commonly employed a tagging technique known as visible implant elastomer (VIE). This method has not been widely adopted by the zebrafish research community and also lacks refinement with regard to animal welfare. The current paper introduces a new VIE tagging protocol, with the aim of improving existing tagging techniques by placing particular emphasis on the Three Rs. To improve animal welfare and fish survival, we added the use of an analgesic compound (lidocaine) through the marking procedure, followed by after-treatment with antiseptics (melaleuca, aloe vera, and PVP-I as active ingredients) to improve tissue regeneration and healing. The newly improved protocol has been quantitatively evaluated on different populations and age groups of zebrafish. This study will be useful to the scientific zebrafish community and to the wider field including biologist and aquarists, especially in consideration of animal welfare, where tagging techniques are considered as a potential noxious stimulus for fish.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Crespel, Dr Amelie and Killen, Professor Shaun and Thambithurai, Davide and Allan, Ms Brooke and Dwyer, Ms Toni and Racz, Miss Anita
Authors: Rácz, A., Allan, B., Dwyer, T., Thambithurai, D., Crespel, A., and Killen, S. S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Animals
Publisher:MDPI
ISSN:2076-2615
ISSN (Online):2076-2615
Published Online:26 February 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Animals 11(3): 616
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
167015The Influence of Individual Physiology on Group Behaviour in Fish SchoolsShaun KillenNatural Environment Research Council (NERC)NE/J019100/1Institute of Biodiversity, Animal Health and Comparative Medicine