Hu, C. , Kalsi, S., Zeimpekis, I., Sun, K., Ashburn, P., Turner, C., Sutton, J. M. and Morgan, H. (2017) Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors. Biosensors and Bioelectronics, 96, pp. 281-287. (doi: 10.1016/j.bios.2017.05.016) (PMID:28505562)
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Abstract
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.
| Item Type: | Articles |
|---|---|
| Additional Information: | The authors would like to acknowledge the Technology Strategy Board (TSB) and the Engineering and Physical Sciences Research Council (EPSRC, Grant EP/K502327/1) for funding this work. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hu, Dr Chunxiao |
| Authors: | Hu, C., Kalsi, S., Zeimpekis, I., Sun, K., Ashburn, P., Turner, C., Sutton, J. M., and Morgan, H. |
| College/School: | College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering |
| Journal Name: | Biosensors and Bioelectronics |
| Publisher: | Elsevier |
| ISSN: | 0956-5663 |
| ISSN (Online): | 1873-4235 |
| Published Online: | 09 May 2017 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Biosensors and Bioelectronics 96: 281-287 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Data DOI: | 10.5258/SOTON/404101 |
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