Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

Botcherby, E. J., Smith, C. W., Kohl, M. M. , Débarre, D., Booth, M. J., Juškaitis, R., Paulsen, O. and Wilson, T. (2012) Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. Proceedings of the National Academy of Sciences of the United States of America, 109(8), pp. 2919-2924. (doi: 10.1073/pnas.1111662109) (PMID:22315405) (PMCID:PMC3286923)

Abstract

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kohl, Dr Michael
• Authors: Botcherby, E. J., Smith, C. W., Kohl, M. M., Débarre, D., Booth, M. J., Juškaitis, R., Paulsen, O., and Wilson, T.
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
• Journal Name: Proceedings of the National Academy of Sciences of the United States of America
• Publisher: National Academy of Sciences
• ISSN: 0027-8424
• ISSN (Online): 1091-6490
• Published Online: 06 February 2012