A 3D hydrodynamic flow-focusing device for cell sorting

Yuan, X. , Glidle, A., Furusho, H. and Yin, H. (2021) A 3D hydrodynamic flow-focusing device for cell sorting. Microfluidics and Nanofluidics, 25(3), 23. (doi: 10.1007/s10404-021-02425-y)

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Optical-based microfluidic cell sorting has become increasingly attractive for applications in life and environmental sciences due to its ability of sophisticated cell handling in flow. The majority of these microfluidic cell sorting devices employ two-dimensional fluid flow control strategies, which lack the ability to manipulate the position of cells arbitrarily for precise optical detection, therefore resulting in reduced sorting accuracy and purity. Although three-dimensional (3D) hydrodynamic devices have better flow-focusing characteristics, most lack the flexibility to arbitrarily position the sample flow in each direction. Thus, there have been very few studies using 3D hydrodynamic flow focusing for sorting. Herein, we designed a 3D hydrodynamic focusing sorting platform based on independent sheath flow-focusing and pressure-actuated switching. This design offers many advantages in terms of reliable acquisition of weak Raman signals due to the ability to precisely control the speed and position of samples in 3D. With a proof-of-concept demonstration, we show this 3D hydrodynamic focusing-based sorting device has the potential to reach a high degree of accuracy for Raman activated sorting.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Furusho, Dr Hitoshi and Yin, Professor Huabing and Yuan, Dr Xiaofei and Glidle, Dr Andrew
Authors: Yuan, X., Glidle, A., Furusho, H., and Yin, H.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Microfluidics and Nanofluidics
ISSN (Online):1613-4990
Published Online:15 February 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Microfluidics and Nanofluidics 25(3): 23
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172975Stable Isotope Probing with Resonance Raman Cell Sorting to profile influence of ocean acidification on microbial carbon fixationHuabing YinNatural Environment Research Council (NERC)NE/P003826/1ENG - Biomedical Engineering
304198LinkPI: Linking Phenotype function with Identity: a novel integrated single-cell technology and metagenomics approachHuabing YinNatural Environment Research Council (NERC)NE/S008721/1ENG - Biomedical Engineering