Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging

Baibek, A., Üçüncü, M., Short, B., Ramage, G. , Lilienkampf, A. and Bradley, M. (2021) Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging. Chemical Communications, 57(15), pp. 1899-1902. (doi: 10.1039/D0CC08177A) (PMID:33491716)

Abstract

The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.

• Item Type: Articles
• Additional Information: The authors would like to thank the Engineering and Physical Sciences Research Council (UKRI, EPSRC, UK) Interdisciplinary Research Collaboration grants EP/K03197X/1 and EP/R005257/1 for funding.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ramage, Professor Gordon
• Authors: Baibek, A., Üçüncü, M., Short, B., Ramage, G., Lilienkampf, A., and Bradley, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: Chemical Communications
• Publisher: Royal Society of Chemistry
• ISSN: 1359-7345
• ISSN (Online): 1364-548X
• Published Online: 19 January 2021
• Copyright Holders: Copyright © 2021 The Royal Society of Chemistry
• First Published: First published in Chemical Communications 57(15): 1899-1902
• Publisher Policy: Reproduced under a Creative Commons License